Gene-Trac® Environmental Molecular Testing
Gene-Trac® testing is used to assess bioremediation potential and monitor enhanced bioremediation performance by quantifying and characterizing key dechlorinating bacteria in groundwater and soil/sediment from contaminated sites.
Use Gene-Trac® to:
- Determine if suitable microorganisms are present for MNA remedies.
- Predict the effectiveness of biostimulation before the addition of electron donor.
- Determine the need for bioaugmentation.
- Predict if intermediates such as cis-DCE or vinyl chloride are likely to accumulate.
- Validate the performance of enhanced bioremediation projects.
Quantify and Characterize Environmental Microorganisms
Dehalococcoides (Dhc), Dehalobacter (Dhb) and Dehalogenimonas (Dhg) bacteria and key functional genes such as vinyl chloride reductase (vcrA) are critical to bioremediation of many chlorinated compounds, including chlorinated ethenes, ethanes, methanes and propanes. Gene-Trac® quantitative polymerase chain reaction (qPCR) testing is used to characterize and quantify these key dechlorinating bacteria at contaminated sites.
Gene-Trac® Tests Available
| Test | Target | Relevance |
|---|---|---|
| Gene-Trac® Dhc** | Dehalococcoides (Dhc) | Critical to dechlorination of chlorinated ethenes such as PCE and TCE |
| Gene-Trac® VC* | VC-reductase (vcrA) | Key Dhc gene that dechlorinates vinyl chloride to ethene |
| Functional Genes | bVCA/TceA | Functional genes relevant to chlorinated ethene dechlorination |
| Gene-Trac® Dhb | Dehalobacter (Dhb) | Critical in chlorinated methane (CF, DCM) and chlorinated ethane (e.g., 1,1,1-TCA) degradation |
| Gene-Trac® Dhg | Dehalogenimonas (Dhg) | Dechlorinate chlorinated propanes and 1,2-DCA |
| Gene-Trac® Universal | All Bacteria | For determining total bacterial biomass |
